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用减毒狂犬病病毒感染单核细胞或未成熟树突状细胞（DCs）会导致DC成熟以及NFκB信号通路的强烈激活。

Infection of monocytes or immature dendritic cells (DCs) with an attenuated rabies virus results in DC maturation and a strong activation of the NFkappaB signaling pathway.

作者信息

Li Jianwei, McGettigan James P, Faber Milosz, Schnell Matthias J, Dietzschold Bernhard

机构信息

Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA, USA.

出版信息

Vaccine. 2008 Jan 17;26(3):419-26. doi: 10.1016/j.vaccine.2007.10.072. Epub 2007 Nov 29.

DOI:10.1016/j.vaccine.2007.10.072
PMID:18082293
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2185713/
Abstract

To assess the potential role of dendritic cells (DCs) or monocytes in the development of a protective immune response, we infected human immature DCs or monocytes with a live rabies virus (RV) vaccine strain (SPBNGAS-GAS) and a pathogenic RV (DOG4). Both cell types were infected with SPBNGAS-GAS and DOG4 and both RVs were similarly potent in inducing maturation of immature DCs or monocytes. However, in contrast to DOG4, SPBNGAS-GAS induced very high levels of IFN-alpha1 mRNA in monocytes and DCs. Furthermore, at least 26 other genes related to the NFkappaB signaling pathway were strongly upregulated in SPBNGAS-GAS-infected DCs, but only somewhat increased in DOG4-infected cells. Thus, the extent of upregulation of NFkappaB pathway-related genes in DCs infected with the live RV vaccine strain might explain the strong protective activity of SPBNGAS-GAS.

摘要

为评估树突状细胞（DCs）或单核细胞在保护性免疫反应发展中的潜在作用，我们用一种活狂犬病病毒（RV）疫苗株（SPBNGAS-GAS）和一种致病性RV（DOG4）感染人未成熟DCs或单核细胞。两种细胞类型均被SPBNGAS-GAS和DOG4感染，且两种RV在诱导未成熟DCs或单核细胞成熟方面同样有效。然而，与DOG4不同，SPBNGAS-GAS在单核细胞和DCs中诱导出非常高水平的IFN-α1 mRNA。此外，至少26个与NFκB信号通路相关的其他基因在被SPBNGAS-GAS感染的DCs中强烈上调，但在被DOG4感染的细胞中仅略有增加。因此，被活RV疫苗株感染的DCs中NFκB通路相关基因上调的程度可能解释了SPBNGAS-GAS的强大保护活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1a/2185713/de5b161407f8/nihms-38622-f0006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1a/2185713/04534f7d1be4/nihms-38622-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1a/2185713/89206b26b39d/nihms-38622-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1a/2185713/8d40f04129a8/nihms-38622-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1a/2185713/48190f69b6ce/nihms-38622-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1a/2185713/504b73743b8a/nihms-38622-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1a/2185713/de5b161407f8/nihms-38622-f0006.jpg

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