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腺相关病毒7型的生产、纯化及初步X射线晶体学研究

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7.

作者信息

Quesada Odayme, Gurda Brittney, Govindasamy Lakshmanan, McKenna Robert, Kohlbrenner Erik, Aslanidi George, Zolotukhin Sergei, Muzyczka Nicholas, Agbandje-McKenna Mavis

机构信息

Department of Biochemistry and Molecular Biology, McKnight Brain Institute, Center for Structural Biology, University of Florida, Gainesville, FL 32610, USA.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt 12):1073-6. doi: 10.1107/S1744309107060289. Epub 2007 Nov 30.

DOI:10.1107/S1744309107060289
PMID:18084098
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2344100/
Abstract

Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids diffract X-rays to approximately 3.0 A resolution. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = 252.4, c = 591.2 A in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R(merge) of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation-function searches and is positioned at (0, 0, 0) by packing considerations.

摘要

杆状病毒表达的腺相关病毒7型衣壳晶体对X射线的衍射分辨率约为3.0埃。这些晶体属于菱面体空间群R3，在六方晶系中晶胞参数a = 252.4，c = 591.2埃。衍射数据经过处理和归约后，总体完整性为79.0%，R(merge)为12.0%。晶胞中有三个病毒衣壳。二十面体三重轴与晶体学三重轴重合，导致每个晶体学不对称单元中有三分之一的衣壳（20个单体）。病毒衣壳的取向已通过旋转函数搜索确定，并通过堆积考虑定位在(0, 0, 0)处。