Ci-VSP中的亚基组织与功能转变

Subunit organization and functional transitions in Ci-VSP.

作者信息

Kohout Susy C, Ulbrich Maximilian H, Bell Sarah C, Isacoff Ehud Y

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

出版信息

Nat Struct Mol Biol. 2008 Jan;15(1):106-8. doi: 10.1038/nsmb1320. Epub 2007 Dec 16.

DOI:10.1038/nsmb1320

PMID:18084307

Abstract

Voltage-sensing domains (VSDs) confer voltage dependence on effector domains of membrane proteins. Ion channels use four VSDs to control a gate in the pore domain, but in the recently discovered phosphatase Ci-VSP, the number of subunits has been unknown. Using single-molecule microscopy to count subunits and voltage clamp fluorometry to detect structural dynamics, we found Ci-VSP to be a monomer, which operates independently, but nevertheless undergoes multiple voltage-dependent conformational transitions.

摘要

电压传感结构域（VSDs）赋予膜蛋白效应结构域电压依赖性。离子通道利用四个VSDs来控制孔道结构域中的一个门控，但在最近发现的磷酸酶Ci-VSP中，亚基数量一直未知。我们使用单分子显微镜来计数亚基，并通过电压钳荧光法来检测结构动力学，发现Ci-VSP是一种单体，其独立运作，但仍会经历多种电压依赖性构象转变。