Joshi Anjali U, Hinsu Ankit T, Kotadiya Rohitkumar J, Rank Jalpa K, Andharia Kavan N, Kothari Ramesh K
UGC-CAS Department of Biosciences, Saurashtra University, Rajkot, Gujarat 360005 India.
3 Biotech. 2020 May;10(5):214. doi: 10.1007/s13205-020-02205-5. Epub 2020 Apr 25.
Textile industry is one of the anthropogenic activities that consume a large amount of water and pollute water bodies. It uses a massive amount of dyes, which is one of the main constituents of polluting textile effluent. In the present research, biodegradation of Acid Blue 113 dye, a commonly used textile di-azo dye, has been studied exploiting , strain AK6. The dye (300 ppm) was decolorized up to 86.2% within 96 h. The metabolites of Acid Blue 113 obtained after biodegradation were identified by various analytical techniques viz. HPLC (high-performance liquid chromatography) and GC-MS (gas chromatography-mass spectrometry). Genome analysis of isolate AK6 using IMG/M (Integrated Microbial Genomes and Microbiomes) system supported the role of azoreductase and laccase for the decolorization and degradation of azo dye. The ability of AK6 to tolerate high amount of dye makes it a potential candidate for bioremediation and pre-processing to remove dyes from textile effluents.
纺织工业是消耗大量水资源并污染水体的人为活动之一。它使用大量染料，而染料是纺织废水污染的主要成分之一。在本研究中，利用菌株AK6对一种常用的纺织双偶氮染料酸性蓝113进行了生物降解研究。该染料（300 ppm）在96小时内脱色率高达86.2%。通过各种分析技术，即高效液相色谱（HPLC）和气相色谱-质谱联用（GC-MS），对生物降解后获得的酸性蓝113代谢产物进行了鉴定。使用IMG/M（综合微生物基因组和微生物群落）系统对分离株AK6进行基因组分析，支持了偶氮还原酶和漆酶在偶氮染料脱色和降解中的作用。AK6耐受大量染料的能力使其成为生物修复和预处理以去除纺织废水中染料的潜在候选者。
