Biodegradation of azo dye-containing wastewater by activated sludge: a critical review.

The effluent from the textile industry is a complex mixture of recalcitrant molecules that can harm the environment and human health. Biological treatments are usually applied for this wastewater, particularly activated sludge, due to its high efficiency, and low implementation and operation costs. However, the activated sludge microbiome is rarely well-known. In general, activated sludges are composed of Acidobacteria, Bacillus, Clostridium, Pseudomonas, Proteobacteria, and Streptococcus, in which Bacillus and Pseudomonas are highlighted for bacterial dye degradation. Consequently, the process is not carried out under optimum conditions (treatment yield). Therefore, this review aims to contextualize the potential environmental impacts of azo dye-containing wastewater from the textile industry, including toxicity, activated sludge microbiome identification, in particular using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a novel, rapid and accurate strategy for the identification of activated sludge microbiome (potential to enhance treatment yield).