用偏振光分析微管动力学。
Analysis of microtubule dynamics by polarized light.
作者信息
Oldenbourg Rudolf
机构信息
Marine Biological Laboratory, Woods Hole, MA, USA.
出版信息
Methods Mol Med. 2007;137:111-23. doi: 10.1007/978-1-59745-442-1_8.
Abstract
In this article, the author describes the needed instrumentation and the methods to be followed for the observation and measurement of the birefringence of single and bundled microtubules and of their ordered arrays using a polarizing microscope. As instruments, the traditional polarizing microscope and the recently developed LC-PolScope are discussed. As methods, we describe qualitative and quantitative observations, including notes on specimen preparations, which optimize the sensitivity and accuracy of measuring specimen retardance.
摘要
在本文中，作者描述了使用偏光显微镜观察和测量单根及成束微管及其有序阵列的双折射所需的仪器设备和应遵循的方法。作为仪器，讨论了传统的偏光显微镜和最近开发的液晶偏光显微镜（LC-PolScope）。作为方法，我们描述了定性和定量观察，包括关于标本制备的说明，这些说明优化了测量标本延迟的灵敏度和准确性。
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