Wang Xiaomeng, Ellis Jonathan S, Kan Chung-Dann, Li Ren-Ke, Thompson Michael
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Canada M5S 3H6.
Analyst. 2008 Jan;133(1):85-92. doi: 10.1039/b714210b. Epub 2007 Nov 28.
The attachment of rat aortic smooth muscle cells to various surfaces has been monitored by a thickness shear mode acoustic wave device incorporated into an on-line configuration. Using the total injection analysis method, laminin and fibronectin were adsorbed to the device surface, to be followed by introduction of cells into the system. The results of these experiments in terms of frequency and motional resistance measurements were also compared with those for cell attachment to the bare gold electrode of the sensor. The responses of the surface-bound cells to the introduction of various ions, depolarisation events and damage subsequent to exposure to hydrogen peroxide were also observed. Morphological changes in the cells, as confirmed by scanning electron microscopy, are correlated with results of the acoustic wave measurements.
通过在线配置的厚度剪切模式声波装置监测大鼠主动脉平滑肌细胞在各种表面的附着情况。采用全注射分析法，将层粘连蛋白和纤连蛋白吸附到装置表面，随后将细胞引入系统。还将这些实验在频率和运动阻力测量方面的结果与细胞附着在传感器裸金电极上的结果进行了比较。还观察了表面结合细胞对各种离子引入、去极化事件以及暴露于过氧化氢后的损伤的反应。扫描电子显微镜证实的细胞形态变化与声波测量结果相关。
