Zhai You-Gang, Lv Xin-Jun, Sun Xiao-Hong, Fu Shi-Hong, Gong Zheng-da, Fen Yun, Tong Su-Xiang, Wang Zhao-Xiao, Tang Qing, Attoui Houssam, Liang Guo-Dong
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Ying Xin Street, Beijing 100052, PR China.
Department of Arbovirus and Chlamydia, Yunnan Institute of Endemic Diseases Control and Prevention, 33 Wenhua Road, Dali City 67100, Yunnan Province, PR China.
J Gen Virol. 2008 Jan;89(Pt 1):195-199. doi: 10.1099/vir.0.83221-0.
During an investigation of arboviruses in China, a novel densovirus (DNV) was isolated from the adult female Culex pipiens pallens. The virus, designated Culex pipiens pallens densovirus (CppDNV), caused cytopathic effect in C6/36 cells. The virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. The complete coding region of CppDNV was found to be 3335 nt and it contained three open reading frames (ORFs). CppDNV shares 82-93 % identical nucleotides with isolates of the Aedes albopictus densovirus [isolates AalDNV-1, AalDNV-2 (C6/36 DNV) and AalDNV-3], Aedes aegypti densovirus (AaeDNV) and Haemagogus equines densovirus (HeDNV). The nucleotide sequence identity among CppDNV isolates exceeds 98 %. Phylogenetic trees based on non-structural (NS1 and NS2) and capsid (VP) genes show that CppDNV clustered with the species AaeDNV and represents a novel variant of this species within the genus Brevidensovirus.
在中国进行虫媒病毒调查期间，从成年雌性淡色库蚊中分离出一种新型浓核病毒（DNV）。该病毒被命名为淡色库蚊浓核病毒（CppDNV），可在C6/36细胞中引起细胞病变效应。病毒粒子呈二十面体，无包膜，平均直径为24纳米。CppDNV的完整编码区为3335个核苷酸，包含三个开放阅读框（ORF）。CppDNV与白纹伊蚊浓核病毒分离株[分离株AalDNV-1、AalDNV-2（C6/36 DNV）和AalDNV-3]、埃及伊蚊浓核病毒（AaeDNV）和马血库蚊浓核病毒（HeDNV）的核苷酸同一性为82%-93%。CppDNV分离株之间的核苷酸序列同一性超过98%。基于非结构（NS1和NS2）和衣壳（VP）基因构建的系统发育树表明，CppDNV与AaeDNV物种聚类，代表了短浓核病毒属内该物种的一个新变种。
