Giovannini M G, Pieraccini G, Moneti G
Dipartimento di Farmacologia, Università degli Studi, Firenze.
Ann Ist Super Sanita. 1991;27(3):401-10.
The application of gas chromatography-mass spectrometry (GC-MS) for the quantitative analysis of endogenous compounds as well as drugs in clinical biochemistry is taken into account. For this purpose, it is of the utmost importance the choice of a good internal standard (IS). It is demonstrated that the best IS in GC-MS is a stable isotope-labelled analogue of the analyte. In this case only we can speak of isotope dilution mass spectrometry. All the advantages and disadvantages inherent to the use of such a molecule as IS are discussed. The use of a calibration curve to perform the quantitative analysis is described, together with the criteria applied for the definition of the reliability of a method (precision, accuracy and limit of quantification). Examples taken from the literature are described.
考虑了气相色谱-质谱联用（GC-MS）在临床生物化学中对内源性化合物以及药物进行定量分析的应用。为此，选择一种良好的内标（IS）至关重要。结果表明，GC-MS中最佳的内标是分析物的稳定同位素标记类似物。只有在这种情况下，我们才能谈及同位素稀释质谱法。讨论了使用此类分子作为内标所固有的所有优缺点。描述了使用校准曲线进行定量分析的方法，以及用于定义方法可靠性的标准（精密度、准确度和定量限）。还介绍了文献中的实例。
