Srithanaviboonchai Kriengkrai, Rungruengthanakit Kittipong, Nouanthong Phonethipsavanh, Pata Supansa, Sirisanthana Thira, Kasinrerk Watchara
Department of Community Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
J Acquir Immune Defic Syndr. 2008 Feb 1;47(2):135-9. doi: 10.1097/QAI.0b013e3181624ab5.
Several CD4 lymphocyte enumeration methods have been developed to be used as alternatives to conventional flow cytometry. However, no consensus has been reached on which technique should be widely adopted. We introduce here a novel nonflow cytometric method, called CD4 Select, for enumeration of CD4 lymphocytes. Instead of using a flow cytometer, the method requires only an automatic hematoanalyzer (complete blood count [CBC] machine). The correlations of the percentage of CD4 lymphocytes and absolute CD4 counts with the CD4 Select method and standard flow cytometry were 0.932 and 0.922, respectively. The CD4 Select method yielded percentage of CD4 lymphocytes greater than flow cytometry by a mean of 0.06%, and the limits of agreement at 95% confidence interval (CI) were 9.46% to -9.56% using Bland-Altman analysis. The CD4 select method is cheap, valid, and simple enough to be conducted locally at small facilities. The method yields not only absolute CD4 lymphocytes, but also percentage of CD4 lymphocytes. The use of a hematoanalyzer also gives this method relatively high sample throughput and makes it less labor-intensive than other nonflow cytometric methods.
已经开发出几种CD4淋巴细胞计数方法，作为传统流式细胞术的替代方法。然而，对于应广泛采用哪种技术尚未达成共识。我们在此介绍一种名为CD4 Select的新型非流式细胞术方法，用于计数CD4淋巴细胞。该方法无需使用流式细胞仪，仅需一台自动血液分析仪（全血细胞计数[CBC]仪）。CD4淋巴细胞百分比和绝对CD4计数与CD4 Select方法和标准流式细胞术的相关性分别为0.932和0.922。CD4 Select方法得出的CD4淋巴细胞百分比比流式细胞术平均高0.06%，使用Bland-Altman分析，95%置信区间（CI）的一致性界限为9.46%至-9.56%。CD4 Select方法价格便宜、有效且足够简单，可在小型机构本地进行。该方法不仅能得出绝对CD4淋巴细胞数量，还能得出CD4淋巴细胞百分比。使用血液分析仪也使该方法具有相对较高的样本通量，且比其他非流式细胞术方法劳动强度更低。
