Ribel-Madsen S, Christgau S, Gronemann S T, Bartels E M, Danneskiold-Samsøe B, Bliddal H
The Parker Institute, Department of Rheumatology, Frederiksberg Hospital, Capital Region of Denmark, DK-2000 Frederiksberg, Denmark.
Scand J Rheumatol. 2007 Nov-Dec;36(6):470-7. doi: 10.1080/03009740701483048.
To assess the metabolism of collagen in fibromyalgia (FM) patients, and to compare the occurrence of collagen metabolism markers to the severity of FM symptoms.
Morning urine was collected from 27 FM women fulfilling the American College of Rheumatology (ACR) criteria for FM, and from seven controls. FM patients completed the Fibromyalgia Impact Questionnaire (FIQ). Bone mineral density (BMD), isokinetic muscle strength in knee and elbow, and hand-grip strength were measured. Urinary concentrations of collagen type I cross-linked C-telopeptide (CTX-I) and collagen type II cross-linked C-telopeptide (CTX-II) were determined by enzyme-linked immunosorbent assay (ELISA). Pyridinoline (Pyd) and deoxypyridinoline (Dpd) were determined by liquid chromatography, and hydroxyproline (Hyp) by spectrophotometry. All concentration data were normalized to creatinine.
Mean values in the FM group and the control group, respectively, were: urinary CTX-I 246.8 and 337.5 microg/mmol (p = 0.060); CTX-II 110.4 and 185.1 ng/mmol (p = 0.035); Pyd 56.1 and 52.3 nmol/mmol (NS); Dpd 15.1 and 14.0 nmol/mmol (NS); Pyd : Dpd ratio 4.05 and 3.96 (NS); Hyp 26.1 and 21.1 micromol/mmol (NS). Significant inverse correlations were seen between CTX-I and the intensity of fatigue, and between CTX-II and anxiety. An inverse correlation between CTX-I and muscle strength was apparent, but relied on extreme values from one patient, and no significant correlation was found between CTX-I or CTX-II and tender points or BMD in the FM group.
Low urinary concentrations of CTX-II and CTX-I and normal levels of Pyd and Dpd were found in FM, but their relationship to the intensity of FM symptoms was unclear.
评估纤维肌痛（FM）患者的胶原蛋白代谢情况，并比较胶原蛋白代谢标志物的出现情况与FM症状严重程度。
收集27名符合美国风湿病学会（ACR）FM标准的女性FM患者及7名对照者的晨尿。FM患者完成纤维肌痛影响问卷（FIQ）。测量骨密度（BMD）、膝关节和肘关节等速肌力以及握力。采用酶联免疫吸附测定（ELISA）法测定尿中I型胶原交联C端肽（CTX-I）和II型胶原交联C端肽（CTX-II）的浓度。采用液相色谱法测定吡啶啉（Pyd）和脱氧吡啶啉（Dpd），采用分光光度法测定羟脯氨酸（Hyp）。所有浓度数据均以肌酐进行标准化。
FM组和对照组的平均值分别为：尿CTX-I 246.8和337.5μg/mmol（p = 0.060）；CTX-II 110.4和185.1 ng/mmol（p = 0.035）；Pyd 56.1和52.3 nmol/mmol（无显著性差异）；Dpd 15.1和14.0 nmol/mmol（无显著性差异）；Pyd:Dpd比值4.05和3.96（无显著性差异）；Hyp 26.1和21.1μmol/mmol（无显著性差异）。CTX-I与疲劳强度之间以及CTX-II与焦虑之间存在显著的负相关。CTX-I与肌肉力量之间存在明显的负相关，但依赖于一名患者的极值，且在FM组中未发现CTX-I或CTX-II与压痛点或BMD之间存在显著相关性。
FM患者尿中CTX-II和CTX-I浓度较低，Pyd和Dpd水平正常，但其与FM症状强度的关系尚不清楚。
