Department of General Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China.
Hepatobiliary Pancreat Dis Int. 2010 Feb;9(1):83-7.
Severe acute pancreatitis (SAP) is a commonly seen acute abdominal syndrome characterized by sudden onset, rapid progression and high mortality rate. The damage in peripheral organs may be more severe than that in the pancreas, and can even lead to multiple organ dysfunction. It is critical to recognize early pathological changes in multiple organs. This study aimed to assess the early pathological features of damaged organs in a rat model of SAP.
Thirty clean grade healthy male Sprague-Dawley rats weighing 250-300 g were randomly divided into a model control group (n=15) and a sham-operated group (n=15). The SAP rat model was induced by sodium taurocholate. Samples of blood and from multiple organs were collected 3 hours after operation. We assessed the levels of IL-6, TNF-alpha, PLA2, NO, ET-1, MDA, amylases and endotoxin in blood and observed the early pathological changes in multiple damaged organs.
Levels of IL-6, TNF-alpha, PLA2, NO, ET-1 and MDA in serum and of amylase and endotoxin in plasma of the model control group rats were significantly higher than those of the sham-operated group (P<0.01). Different degrees of pathological change were observed in multiple damaged organs.
Multiple organ injury may occur at the early stage of SAP in rats.
重症急性胰腺炎（SAP）是一种常见的急性腹综合征，其特点是起病突然、进展迅速、死亡率高。外周器官的损伤可能比胰腺更严重，甚至可导致多器官功能障碍。早期识别多个器官的病理变化至关重要。本研究旨在评估 SAP 大鼠模型中受损器官的早期病理特征。
将 30 只清洁级健康雄性 Sprague-Dawley 大鼠（体重 250-300 g）随机分为模型对照组（n=15）和假手术组（n=15）。采用牛磺胆酸钠诱导 SAP 大鼠模型。术后 3 小时采集血液和多个器官样本。检测血清中白细胞介素 6（IL-6）、肿瘤坏死因子-α（TNF-α）、磷脂酶 A2（PLA2）、一氧化氮（NO）、内皮素-1（ET-1）、丙二醛（MDA）、淀粉酶和内毒素水平，观察多个受损器官的早期病理变化。
模型对照组大鼠血清中 IL-6、TNF-α、PLA2、NO、ET-1 和 MDA 水平以及血浆中淀粉酶和内毒素水平均明显高于假手术组（P<0.01）。多个受损器官出现不同程度的病理变化。
SAP 大鼠早期可能发生多器官损伤。
