γ-氨基丁酸A型受体RDL抑制果蝇嗅觉联想学习。
GABAA receptor RDL inhibits Drosophila olfactory associative learning.
作者信息
Liu Xu, Krause William C, Davis Ronald L
机构信息
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
出版信息
Neuron. 2007 Dec 20;56(6):1090-102. doi: 10.1016/j.neuron.2007.10.036.
In both mammals and insects, neurons involved in learning are strongly modulated by the inhibitory neurotransmitter GABA. The GABAA receptor, resistance to dieldrin (Rdl), is highly expressed in the Drosophila mushroom bodies (MBs), a group of neurons playing essential roles in insect olfactory learning. Flies with increased or decreased expression of Rdl in the MBs were generated. Olfactory associative learning tests showed that Rdl overexpression impaired memory acquisition but not memory stability. This learning defect was due to disrupting the physiological state of the adult MB neurons rather than causing developmental abnormalities. Remarkably, Rdl knockdown enhanced memory acquisition but not memory stability. Functional cellular imaging experiments showed that Rdl overexpression abolished the normal calcium responses of the MBs to odors while Rdl knockdown increased these responses. Together, these data suggest that RDL negatively modulates olfactory associative learning, possibly by gating the input of olfactory information into the MBs.
在哺乳动物和昆虫中，参与学习的神经元都受到抑制性神经递质γ-氨基丁酸（GABA）的强烈调节。抗狄氏剂的γ-氨基丁酸A型受体（Rdl）在果蝇蘑菇体（MBs）中高度表达，蘑菇体是一组在昆虫嗅觉学习中起关键作用的神经元。我们培育出了蘑菇体中Rdl表达增加或减少的果蝇。嗅觉联想学习测试表明，Rdl过表达会损害记忆获取，但不会影响记忆稳定性。这种学习缺陷是由于破坏了成年蘑菇体神经元的生理状态，而不是导致发育异常。值得注意的是，敲低Rdl可增强记忆获取，但不影响记忆稳定性。功能性细胞成像实验表明，Rdl过表达消除了蘑菇体对气味的正常钙反应，而敲低Rdl则增加了这些反应。总之，这些数据表明，RDL可能通过控制嗅觉信息进入蘑菇体的输入，对嗅觉联想学习产生负调节作用。
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