用于检测呼吸道感染的多重实时聚合酶链反应
Multiplex real-time PCR for detection of respiratory tract infections.
作者信息
Brittain-Long Robin, Nord Sandra, Olofsson Sigvard, Westin Johan, Anderson Lars-Magnus, Lindh Magnus
机构信息
Department of Infection, the Sahlgrenska Academy, Göteborg University, 416 85 Göteborg, Sweden.
出版信息
J Clin Virol. 2008 Jan;41(1):53-6. doi: 10.1016/j.jcv.2007.10.029.
Abstract
BACKGROUND
Broad diagnostics of respiratory infection by molecular assays has not yet won acceptance due to technical difficulties and high costs.
OBJECTIVES
To evaluate clinical applicability of multiplex real-time PCR.
STUDY DESIGN
An assay targeting influenza virus A (IfA) and B (IfB), parainfluenza 1-3 (PIV), human metapneumovirus (MPV), respiratory syncytial virus (RSV), rhinovirus (RV), enterovirus (EV), adenovirus (AdV), human coronaviruses (229E, OC43, NL63), M. pneumoniae and Ch. pneumoniae was developed and run daily on consecutive clinical nasopharyngeal swab samples.
RESULTS
An etiology was identified in 48% of the 954 samples, with IfA in 25%, RV in 20%, MPV in 10% and M. pneumoniae in 10% of the positive. By a rational procedure costs could be reduced and the customer price set relatively low (euro33 per sample).
CONCLUSION
Streamlined testing and cost limitation is achievable and probably critical for implementation of a broad molecular diagnostics of respiratory infections.
摘要
背景
由于技术难题和高成本，通过分子检测对呼吸道感染进行广泛诊断尚未得到认可。
目的
评估多重实时聚合酶链反应的临床适用性。
研究设计
开发了一种针对甲型流感病毒（IfA）、乙型流感病毒（IfB）、副流感病毒1 - 3型（PIV）、人偏肺病毒（MPV）、呼吸道合胞病毒（RSV）、鼻病毒（RV）、肠道病毒（EV）、腺病毒（AdV）、人冠状病毒（229E、OC43、NL63）、肺炎支原体和肺炎衣原体的检测方法，并每天对连续的临床鼻咽拭子样本进行检测。
结果
在954份样本中，48%的样本确定了病因，其中甲型流感病毒占阳性样本的25%，鼻病毒占20%，人偏肺病毒占10%，肺炎支原体占10%。通过合理的流程可以降低成本，并将客户价格设定得相对较低（每份样本33欧元）。
结论
简化检测流程和控制成本是可行的，这对于广泛开展呼吸道感染分子诊断可能至关重要。
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