Kazanis Ilias, Belhadi Aisha, Faissner Andreas, Ffrench-Constant Charles
Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom.
J Neurosci. 2007 Dec 19;27(51):13991-6. doi: 10.1523/JNEUROSCI.3279-07.2007.
The subependymal zone (SEZ) of the lateral ventricles of the adult mouse brain hosts neurogenesis from a neural stem cell population with the morphology of astrocytes (termed type-B cells). Tenascin-C is a large extracellular matrix glycoprotein present in the SEZ that has been shown to regulate the development of embryonic neural stem cells and the proliferation and migration of early postnatal neural precursors. Here we show that tenascin-C is produced by type-B cells and forms a layer between SEZ and the adjacent striatum. Tenascin-C deficiency resulted in minor structural differences in and around the SEZ. However, the numbers of neural stem cells and their progeny remained unaffected, as did their regeneration after depletion of mitotic cells using the antimitotic drug cytosine-beta-D-arabinofuranoside. Our results reveal a remarkable ability of the adult neural stem cell niche to retain proper function even after the removal of major extracellular matrix molecules.
成年小鼠脑侧脑室的室管膜下区（SEZ）存在由具有星形胶质细胞形态的神经干细胞群体（称为B型细胞）进行的神经发生。腱生蛋白-C是一种存在于SEZ的大型细胞外基质糖蛋白，已被证明可调节胚胎神经干细胞的发育以及出生后早期神经前体细胞的增殖和迁移。在这里，我们表明腱生蛋白-C由B型细胞产生，并在SEZ和相邻纹状体之间形成一层。腱生蛋白-C缺乏导致SEZ及其周围出现微小的结构差异。然而，神经干细胞及其后代的数量未受影响，使用抗有丝分裂药物β-D-阿拉伯呋喃糖胞苷耗尽有丝分裂细胞后它们的再生情况也是如此。我们的结果揭示了成年神经干细胞生态位即使在去除主要细胞外基质分子后仍具有保持正常功能的显著能力。
