Park Younhee, Song Jaewoo, Song Sungwook, Song Kyung Soon, Ahn Mee Suk, Yang Mi-Sook, Kim Il, Choi Jong Rak
Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.
Korean J Lab Med. 2007 Jun;27(3):162-8. doi: 10.3343/kjlm.2007.27.3.162.
The performance of Cell-Dyn Sapphire (Abbott Diagnostic, USA) was compared to the Bayer Advia 2120 (Bayer Diagnostics, USA), Sysmex XE-2100 (Sysmex Corporation, Japan), and reference microscopy.
Three hundred samples for routine CBC and WBC differentials were randomly chosen for a comparison analysis. The Cell-Dyn Sapphire system was evaluated according to the linearity, imprecision, inter-instrument correlations, and white blood cell differential.
The CBC parameters (WBC, RBC, hemoglobin and platelet) showed a significant linearity with correlation coefficients greater than 0.99 (P<0.0001). Coefficients of variation (CV) for within-run and differential count of WBC were less than 5% except for Total CV for monocytes, eosinophils, and basophils and within-run CV for low valued eosinophils. The correlation coefficients with manual count were lower in monocytes, eosinophils, and basophils than in neutrophils and lymphocytes. The correlation with other hematology anlayzers was significant exclusive of basophils.
These results demonstrate that the Cell-Dyn Sapphire has a good linearity, an acceptable reproducibility, a minimal carryover, and a comparable performance with the sysmex XE-2100 and Advia 2120.
将美国雅培诊断公司的Cell-Dyn Sapphire血细胞分析仪与美国拜耳诊断公司的Bayer Advia 2120、日本Sysmex公司的Sysmex XE-2100以及参考显微镜检查进行性能比较。
随机选取300份用于常规全血细胞计数（CBC）和白细胞分类计数的样本进行比较分析。根据线性度、不精密度、仪器间相关性和白细胞分类对Cell-Dyn Sapphire系统进行评估。
全血细胞计数参数（白细胞、红细胞、血红蛋白和血小板）显示出显著的线性关系，相关系数大于0.99（P<0.0001）。白细胞批内变异系数（CV）和分类计数的CV除单核细胞、嗜酸性粒细胞和嗜碱性粒细胞的总CV以及低值嗜酸性粒细胞的批内CV外均小于5%。单核细胞、嗜酸性粒细胞和嗜碱性粒细胞与手工计数的相关系数低于中性粒细胞和淋巴细胞。除嗜碱性粒细胞外，与其他血液分析仪的相关性显著。
这些结果表明，Cell-Dyn Sapphire具有良好的线性度、可接受的重复性、最小的携带污染，并且与Sysmex XE-2100和Advia 2120性能相当。
