Vives-Corrons J L, Besson I, Jou J M, Gutierrez G
Hematology Laboratory Department, Hospital Clínic i Provincial, University of Barcelona, Spain.
Am J Clin Pathol. 1996 May;105(5):553-9. doi: 10.1093/ajcp/105.5.553.
The Abbott (R) Cell-Dyn 3500 (Abbott CD 3500, Abbott Diagnostics Division, Mountain View, CA) is a fully automated hematology analyzer capable of providing a complete blood count (CBC) profile, including a five-part differential leukocyte count (DLC) and flagging system in this study. The CBC profile and DLC flagging system of the Abbott CD 3500 were evaluated according to the HA-20 protocol of the National Committee for Clinical and Laboratory Standards (NCCLS) and compared to the Technicon H*2 blood analyzer currently used in the authors' laboratory. Linearity, carryover, precision, and stability were all within the limits established by the manufacturer. No significant break-downs were found during the evaluation period. Evaluation of DLC indicated an excellent correlation with the manual reference method for neutrophils, lymphocytes, and eosinophils (r = 0.916, 0.936, 0.967, respectively), a good correlation for monocytes (r = 0.811) and a poor correlation for basophils (r = 0.224). Overall flagging for morphologic abnormalities displayed higher sensitivity (85%) than specificity (67%), with a false-positive ratio of 33%. In general, these results are in accordance with those obtained by other authors in the same period of time.
雅培（R）Cell-Dyn 3500（雅培CD 3500，雅培诊断部，加利福尼亚州山景城）是一台全自动血液分析仪，在本研究中能够提供全血细胞计数（CBC）分析，包括五分类白细胞计数（DLC）和标记系统。根据美国国家临床和实验室标准委员会（NCCLS）的HA-20方案对雅培CD 3500的CBC分析和DLC标记系统进行了评估，并与作者实验室目前使用的Technicon H*2血液分析仪进行了比较。线性、残留、精密度和稳定性均在制造商规定的限度内。在评估期间未发现重大故障。DLC评估表明，中性粒细胞、淋巴细胞和嗜酸性粒细胞与手工参考方法具有极好的相关性（r分别为0.916、0.936、0.967），单核细胞相关性良好（r = 0.811），嗜碱性粒细胞相关性较差（r = 0.224）。形态学异常的总体标记显示出较高的敏感性（85%），但特异性较低（67%），假阳性率为33%。总体而言，这些结果与同期其他作者获得的结果一致。
