Rusznák Zoltán, Bakondi Gábor, Kosztka Lívia, Pocsai Krisztina, Dienes Beatrix, Fodor János, Telek Andrea, Gönczi Mónika, Szucs Géza, Csernoch László
Department of Physiology, Research Centre for Molecular Medicine,Medical and Health Science Centre, University of Debrecen, Debrecen, P.O. Box 22, 4012, Hungary.
Virchows Arch. 2008 Apr;452(4):415-26. doi: 10.1007/s00428-007-0545-x. Epub 2007 Dec 20.
The presence of TASK-3 channels has been described in a number of healthy and malignantly transformed cells, showing mainly intracellular distribution with relatively insignificant labelling of the cell surface membrane. In this work, immunochemical and molecular biology methods were utilised to establish the intracellular organelle whose TASK-3 expression accounts for this strong intracellular labelling using cultured melanoma and HaCaT cells. Before the immunocytochemical experiments, the presence of TASK-3 mRNA was also confirmed in melanoma cells. Comparison of the results of the TASK-3- and mitochondrion-specific labelling indicated that the TASK-3 channel subunits were strongly expressed by mitochondria in both investigated cell types. Moreover, prominent TASK-3 expression of keratinocytes could also be demonstrated in histological sections excised from the human skin. These results indicate that TASK-3 channels are present in the mitochondria in both malignantly transformed and healthy cells, suggesting that they might have roles in ensuring mitochondrial functions.
TASK-3通道已在多种健康细胞和恶性转化细胞中被发现，其主要分布于细胞内，细胞表面膜标记相对较少。在本研究中，利用免疫化学和分子生物学方法，以培养的黑色素瘤细胞和HaCaT细胞为研究对象，确定表达TASK-3从而导致细胞内强标记的细胞内细胞器。在免疫细胞化学实验之前，黑色素瘤细胞中也证实了TASK-3 mRNA的存在。TASK-3特异性标记和线粒体特异性标记结果的比较表明，在两种研究的细胞类型中，线粒体均强烈表达TASK-3通道亚基。此外，从人皮肤切除的组织切片中也可证明角质形成细胞中TASK-3表达显著。这些结果表明，TASK-3通道存在于恶性转化细胞和健康细胞的线粒体中，提示它们可能在确保线粒体功能方面发挥作用。
