Kreshchenko Natalia D, Sedelnikov Zakhar, Sheiman Inna M, Reuter Maria, Maule Aaron G, Gustafsson Margaretha K S
Institute of Cell Biophysics, Pushchino, Moscow Region, Russia.
Cell Tissue Res. 2008 Mar;331(3):739-50. doi: 10.1007/s00441-007-0519-y. Epub 2007 Dec 20.
The effects of neuropeptide F (NPF; from Moniezia expansa) on the regeneration of Girardia tigrina were studied. The animals were decapitated and incubated in water (control) or NPF. The dynamics of the proliferation of the neoblasts in the developing tissue were studied during the course of regeneration by monitoring the mitotic index (MI). The effects of incubation in FMRFamide and GYIRFamide on the MI were also tested. The course of cephalic regeneration was followed with in vivo computer-assisted morphometry for up to 7 days. The development of the regenerating nervous system and the musculature was visualised by immunostaining with a primary antiserum to the C-terminal decapeptide of NPF (YFAIIGRPRFa) and tetramethylrhodamine-isothiocyanate-conjugated phalloidin, which stains F-actin in muscle filaments. The study showed that NPF had a stimulatory effect on the mitotic activity of the neoblasts. FMRFamide and GYIRFamide did not have this effect. NPF also stimulated the growth of the regenerating head and the growing nervous system and musculature. NPF is postulated to have a morphogenetic action in the regenerating animals.
研究了神经肽F（NPF；来自扩展莫尼茨绦虫）对虎纹涡虫再生的影响。将动物断头后置于水中（对照组）或NPF中培养。在再生过程中，通过监测有丝分裂指数（MI）研究发育组织中新生细胞的增殖动态。还测试了在FMRF酰胺和GYIRF酰胺中培养对MI的影响。采用体内计算机辅助形态测量法跟踪头部再生过程长达7天。通过用针对NPF C端十肽（YFAIIGRPRFa）的一抗和异硫氰酸四甲基罗丹明缀合的鬼笔环肽进行免疫染色，观察再生神经系统和肌肉组织的发育，后者可对肌丝中的F-肌动蛋白进行染色。研究表明，NPF对新生细胞的有丝分裂活性具有刺激作用。FMRF酰胺和GYIRF酰胺没有这种作用。NPF还刺激了再生头部以及正在生长的神经系统和肌肉组织的生长。据推测，NPF在再生动物中具有形态发生作用。
