Conrad Dean H, Goyette Jesse, Thomas Paul S
Inflammatory Diseases Research Unit, School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia.
J Gen Intern Med. 2008 Jan;23 Suppl 1(Suppl 1):78-84. doi: 10.1007/s11606-007-0411-1.
The study of expressed proteins in neoplasia is undergoing a revolution with the advent of proteomic analysis. Unlike genomic studies where individual changes may have no functional significance, protein expression is closely aligned with cellular activity. This perspective will review proteomics as a method of detecting markers of neoplasia with a particular emphasis on lung cancer and the potential to sample the lung by exhaled breath condensate (EBC). EBC collection is a simple, new, and noninvasive technique, which allows sampling of lower respiratory tract fluid. EBC enables the study of a wide variety of biological markers from low molecular weight mediators to macromolecules, such as proteins, in a range of pulmonary diseases. EBC may be applied to the detection of lung cancer where it could be a tool in early diagnosis. This perspective will explore the potential of applying proteomics to the EBC from lung cancer patients as an example of detecting potential biomarkers of disease and progression.
随着蛋白质组学分析的出现，肿瘤中表达蛋白的研究正在经历一场变革。与基因组研究不同，在基因组研究中个体变化可能没有功能意义，而蛋白质表达与细胞活性密切相关。本文将综述蛋白质组学作为一种检测肿瘤标志物的方法，特别强调肺癌以及通过呼出气冷凝液（EBC）对肺部进行采样的潜力。EBC采集是一种简单、新颖且无创的技术，它允许对下呼吸道液体进行采样。EBC能够研究从低分子量介质到蛋白质等大分子在内的多种生物标志物，适用于一系列肺部疾病。EBC可应用于肺癌检测，有可能成为早期诊断的工具。本文将以肺癌患者的EBC为例，探讨应用蛋白质组学检测疾病和病情进展潜在生物标志物的潜力。
