Ban Haiqun, Ba Yue, Cheng Xuemin, Wang Guangzhou
School of Public Health, Zhengzhou University, Zhengzhou 450052, China.
Wei Sheng Yan Jiu. 2007 Sep;36(5):532-4.
To investigate the contaminative, condition of planktonic algae, cyanobacteria, toxigenic microcystis and microcystin in Huayuankou Conservation Pool of Yellow River.
From March 2005 to January 2006, water samples were taken 15 times by 2. 5L plastic sampler from Huayuankou Conservation Pool. The density of algae were counted by using blood cell counter. Phycocyanin intergenic spacer region (PC-IGS) and microcystin synthetase gene B (mcyB) of toxigenic microcystis was identified by the whole cell PCR. The concentration of microcystin was determined by ELISA kit. The positive results of PCR and ELISA were compared.
Bacillariophyta, chlorophyta, cyanophyta (cyanobacteria) and euglenophyta were main algaes in Huayuankou conservation pool, and the dominant algae and cell density changed seasonally. Algae cell density and cyanobacteria cell density were higher in summer and autumn than in spring and winter. From July to November, 2005, PC-IGS and mcyB were detected positively by whole cell PCR. Microcystin was positively detected from July, the concentration of microcystin changed from 0 to 0.25microg/L, it was more higher in summer than other seasons.
Toxigenic microcystis and microcystin could be detected in Huayuankou Conservation Pool of Yellow River. Whole cell PCR could be used to identify toxigenic microcystis.
调查黄河花园口蓄水区浮游藻类、蓝藻、产毒微囊藻及微囊藻毒素的污染状况。
2005年3月至2006年1月，用2.5L塑料采样器从黄河花园口蓄水区采集水样15次。采用血细胞计数板计数藻类密度。通过全细胞PCR鉴定产毒微囊藻的藻蓝蛋白基因间隔区（PC-IGS）和微囊藻毒素合成酶基因B（mcyB）。用ELISA试剂盒测定微囊藻毒素浓度。比较PCR和ELISA的阳性结果。
黄河花园口蓄水区主要藻类有硅藻门、绿藻门、蓝藻门（蓝藻）和裸藻门，优势藻类及细胞密度随季节变化。夏秋季节藻类细胞密度和蓝藻细胞密度高于冬春季节。2005年7月至11月，全细胞PCR检测PC-IGS和mcyB呈阳性。7月开始检测到微囊藻毒素呈阳性，微囊藻毒素浓度为0～0.25μg/L，夏季浓度高于其他季节。
黄河花园口蓄水区可检测到产毒微囊藻及微囊藻毒素。全细胞PCR可用于鉴定产毒微囊藻。
