Xu Yao, Wu Zhongxing, Yu Boshi, Peng Xin, Yu Gongliang, Wei Zhihong, Wang Guoxiang, Li Renhui
Institute of Hydrobiology, The Chinese Academy of Sciences, Donghu Nanlu 7, Wuhan, Hubei 430072, PR China.
Environ Pollut. 2008 Nov;156(1):162-7. doi: 10.1016/j.envpol.2007.12.027. Epub 2008 Feb 20.
It is well known that several morphospecies of Microcystis, such as Microcystis aeruginosa (Kützing) Lemmermann and Microcystis viridis (A. Brown) Lemmermann can produce hepatotoxic microcystins. However, previous studies gave contradictory conclusions about microcystin production of Microcystis wesenbergii (Komárek) Komárek. In the present study, ten Microcystis morphospecies were identified in waterblooms of seven Chinese waterbodies, and Microcystis wesenbergii was shown as the dominant species in these waters. More than 250 single colonies of M. wesenbergii were chosen, under morphological identification, to examine whether M. wesenbergii produce hepatotoxic microcystin by using multiplex PCR for molecular detection of a region (mcyA) of microcystin synthesis genes, and chemical analyses of microcystin content by ELISA and HPLC for 21 isolated strains of M. wesenbergii from these waters were also performed. Both molecular and chemical methods demonstrated that M. wesenbergii from Chinese waters did not produce microcystin.
众所周知，微囊藻的几个形态种，如铜绿微囊藻（Kützing）Lemmermann和绿色微囊藻（A. Brown）Lemmermann能够产生肝毒性微囊藻毒素。然而，先前的研究对于惠氏微囊藻（Komárek）Komárek产生微囊藻毒素的情况给出了相互矛盾的结论。在本研究中，在中国七个水体的水华中鉴定出了十种微囊藻形态种，其中惠氏微囊藻在这些水体中为优势种。挑选了250多个惠氏微囊藻单菌落，在形态学鉴定的基础上，通过多重PCR对微囊藻毒素合成基因的一个区域（mcyA）进行分子检测，以检验惠氏微囊藻是否产生肝毒性微囊藻毒素，并且还对从这些水体中分离出的21株惠氏微囊藻进行了ELISA和HPLC微囊藻毒素含量化学分析。分子和化学方法均表明，中国水体中的惠氏微囊藻不产生微囊藻毒素。
