[Respiratory virus expression in renal tissues and urine of children with steroid responsive simple nephrotic syndrome].

OBJECTIVE

To explore the relationship between respiratory virus infection and the episode of steroid responsive simple nephrotic syndrome (SRSNS).

METHODS

Thirty eight children with SRSNS were recruited (28 in the active stage, 10 in the remission stage). Sixty four children (18 with nephritic nephrosis, 16 with bronchiolitis, 15 with secondary glomerular diseases and 15 without diagnosed diseases) served as controls. Reverse transcriptase-PCR (RT-PCR) and alkaline phosphoesterase-anti alkaline phosphoesterase enzyme-linked assay (APAAP) were employed to detect the viral genes and antigens in the urines respectively. The viral antigens in the renal tissues of two children with active SRSNS were also examined by APAAP.

RESULTS

The viruses were more often detected in the urines of children with active SRSNS than those with remission SRSNS and the controls. The respiratory syncytial virus (RSV) was the most common virus detected in the urines of children with active SRSNS. The appearance of the viruses gene and antigens was not influenced by the use of steroid. The same antigens were found in the renal tissues of the two children with active SRSNS.

CONCLUSION

Respiratory tract viruses may play an important role of triggering the SRSNS.