Department of Pediatrics, West China Second University Hospital, Sichuan University, No. 20, Section 3, South Renmin Road, Chengdu, 610041, Sichuan Province, China.
National Center for Birth Defect Monitoring, West China Second University Hospital, Sichuan University, No. 17, Section 3, South Renmin Road, Chengdu, 610041, Sichuan Province, China.
BMC Nephrol. 2023 Feb 16;24(1):36. doi: 10.1186/s12882-023-03083-8.
More attention has been put on the relationship between pediatric glomerular disease and respiratory tract virus infection. Children with glomerular illness, however, are uncommonly found to have biopsy-proven pathological evidence of viral infection. The purpose of this study is to determine whether and what kind of respiratory viruses are found in renal biopsy from glomerular disorders.
We used a multiplex PCR to identify a wide range of respiratory tract viruses in the renal biopsy samples (n = 45) from children with glomerular disorders and a specific PCR to verify their expression.
These case series included 45 of 47 renal biopsy specimens, with 37.8% of male and 62.2% of female patients. Indications for a kidney biopsy were present in all of the individuals. In 80% of the samples, respiratory syncytial virus was discovered. Following that, the RSV subtypes in several pediatric renal disorders were found. There were 16 RSVA positives, 5 RSVB positives, and 15 RSVA/B positives, accounting for 44.4%, 13.9%, and 41.7%, respectively. Nephrotic syndrome samples made up 62.5% of RSVA positive specimens. The RSVA/B-positive was detected in all pathological histological types.
Patients with glomerular disease exhibit respiratory tract viral expression in the renal tissues, especially respiratory syncytial virus. This research offers new information on the detection of respiratory tract viruses in renal tissue, which may facilitate the identification and treatment of pediatric glomerular diseases.
人们越来越关注小儿肾小球疾病与呼吸道病毒感染之间的关系。然而，患有肾小球疾病的儿童通常不会在肾脏活检中发现有病毒感染的病理证据。本研究旨在确定肾小球疾病患者的肾脏活检中是否存在以及存在何种类型的呼吸道病毒。
我们使用多重 PCR 技术在肾小球疾病患儿的肾脏活检样本（n=45）中鉴定了广泛的呼吸道病毒，并使用特定 PCR 技术验证了它们的表达。
该病例系列包括 47 个肾脏活检标本中的 45 个，男性占 37.8%，女性占 62.2%。所有患者均有进行肾活检的指征。80%的样本中发现了呼吸道合胞病毒。随后，在几种儿科肾脏疾病中发现了 RSV 亚型。RSVA 阳性 16 例，RSVB 阳性 5 例，RSVA/B 阳性 15 例，分别占 44.4%、13.9%和 41.7%。肾病综合征样本中 RSVA 阳性标本占 62.5%。RSVA/B 阳性在所有病理组织学类型中均有检出。
肾小球疾病患者的肾脏组织中存在呼吸道病毒表达，尤其是呼吸道合胞病毒。本研究为检测肾脏组织中的呼吸道病毒提供了新信息，可能有助于识别和治疗小儿肾小球疾病。
