Mouso Nora, Diorio Luis, Forchiassin Flavia
Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Rev Iberoam Micol. 2007 Dec 31;24(4):294-8. doi: 10.1016/s1130-1406(07)70059-1.
Stereum hirsutum, a white rot fungus, has a good growth in solid state fermentation. This was carried on with wheat bran, soy bran and a mixture of both. Mycelia grown on soy bran showed the highest decolorization activity on Ponceau 2R (xylidine), indigo carmine and malachite green. Optimal relationship between decolorization and detoxification of malachite green was 30 g of fresh weight (mycelia plus substrate) in 500 ml malachite green solution, 42 U/l of laccase was measured in this solution. Decolorization was carried on without the addition either of nutrients or mediators. Conditions for maximal decolorization did not agree with those for maximal ligninolytic enzyme production, but effectiveness of laccase activity on decolorization was evidenced by electrophoretic analysis, that allowed laccase identification and its decolorization activity in gels stained with indigo carmine and malachite green, with ABTS as mediator. Detoxification was assayed using the sensible fungus Phanerochaete chrysosporium.
毛栓菌，一种白腐真菌，在固态发酵中生长良好。实验采用麦麸、豆粕以及二者的混合物进行。在豆粕上生长的菌丝体对丽春红2R（二甲苯胺）、靛蓝胭脂红和孔雀石绿表现出最高的脱色活性。孔雀石绿脱色与解毒的最佳关系是在500毫升孔雀石绿溶液中加入30克鲜重（菌丝体加底物），该溶液中测得漆酶活性为42 U/升。脱色过程中不添加任何营养物质或介质。最大脱色条件与最大木质素分解酶产生条件不一致，但通过电泳分析证明了漆酶活性对脱色的有效性，电泳分析可在以ABTS为介质、用靛蓝胭脂红和孔雀石绿染色的凝胶中鉴定漆酶及其脱色活性。使用敏感真菌黄孢原毛平革菌测定解毒情况。
