采用心腔双层法保存后作为大鼠心脏移植潜在供体的缺血性损伤心脏。

Ischemically damaged heart after preservation by the cavitary two-layer method as a possible donor in rat heart transplantation.

作者信息

Kamoda Yasuhisa, Fujino Yasuhiro, Tanioka Yasuki, Sakai Tetsuya, Kuroda Yoshikazu

机构信息

Department of Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.

出版信息

J Heart Lung Transplant. 2007 Dec;26(12):1320-5. doi: 10.1016/j.healun.2007.07.037. Epub 2007 Oct 26.

DOI:10.1016/j.healun.2007.07.037

PMID:18096485

Abstract

BACKGROUND

The purpose of this study was to examine the possibility of using an ischemically damaged heart, after preservation by the cavitary two-layer (CTL) method, as a donor in heart transplantation.

METHODS

Each donor heart was heterotopically transplanted to the recipient aorta. The grafts in Group 1 were immediately transplanted. In Group 2, the grafts with (a) 15- or (b) 30-minute warm ischemia were transplanted. The ischemically damaged grafts were transplanted after preservation for 3 hours in University of Wisconsin (UW) solution (Group 3) or CTL (Group 4). Five-day animal survival, tissue adenine triphosphate (ATP) concentration, biochemical assay and histopathologic data were obtained.

RESULTS

Five-day survival in Group 4a was 7 of 8, with significant recovery of the ATP tissue level (9.31 +/- 0.80 micromol/dry weight). Biochemical and pathologic examinations demonstrated that ischemia-reperfusion injury was prevented in Group 4a compared with Group 2a.

CONCLUSIONS

An ischemically damaged rat heart preserved for 3 hours by CTL was found to be a potential donor in rat heart transplantation.

摘要

背景

本研究的目的是探讨采用腔室双层（CTL）法保存后的缺血损伤心脏作为心脏移植供体的可能性。

方法

将每个供体心脏异位移植到受体主动脉。第1组的移植物立即进行移植。第2组中，移植有（a）15分钟或（b）30分钟热缺血的移植物。缺血损伤的移植物在威斯康星大学（UW）溶液中保存3小时后（第3组）或采用CTL法保存后（第4组）进行移植。获取了5天的动物存活率、组织三磷酸腺苷（ATP）浓度、生化分析和组织病理学数据。