Fujino Yasuhiro, Kakinoki Keitaro, Suzuki Yasuyuki, Li Shiri, Tanaka Tomohiro, Tanioka Yasuki, Sakai Tetsuya, Ku Yonson, Kuroda Yoshikazu
Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Japan.
Transplantation. 2003 Sep 15;76(5):777-80. doi: 10.1097/01.TP.0000082526.60737.AE.
The purpose of this study is to examine the possibility of a long-term preservation of the ischemically damaged intestine by the cavitary two-layer method (TLM) in canine small intestinal transplantation.
The grafts were allotransplanted without preservation immediately (group 1) or after 30 minutes of warm ischemia (group 2). The ischemically damaged grafts were also allotransplanted after cold preservation for 24 hours in University of Wisconsin (UW) solution (group 3) or the cavitary TLM (group 4). Seven-day survivals, tissue adenosine triphosphate (ATP) concentrations, absorption tests, and histopathology were examined.
seven-day survivals in groups 1, 2, 3, and 4 were 8 of 8, 6 of 8, 0 of 8, and 6 of 8, respectively. In group 4, significant recovery of ATP tissue level was seen after preservation compared with group 3, and absorption function and regeneration of the graft mucosa recovered at day 14.
Ischemically damaged canine small intestine could be preserved for 24 hours by the cavitary TLM.
本研究的目的是探讨在犬小肠移植中采用腔隙双层法（TLM）长期保存缺血损伤肠段的可能性。
移植物立即进行同种异体移植（未保存，第1组）或在热缺血30分钟后进行同种异体移植（第2组）。缺血损伤的移植物在威斯康星大学（UW）溶液中冷藏保存24小时后（第3组）或采用腔隙TLM保存后（第4组）也进行同种异体移植。检测7天生存率、组织三磷酸腺苷（ATP）浓度、吸收试验和组织病理学。
第1、2、3和4组的7天生存率分别为8/8、6/8、0/8和6/8。在第4组中，与第3组相比，保存后ATP组织水平有显著恢复，移植物黏膜的吸收功能和再生在第14天恢复。
采用腔隙TLM可将缺血损伤的犬小肠保存24小时。
