Tamura H, Mochizuki H, Shigeta M, Arakawa H, Kuroume T
Department of Pediatrics, Gunma University, School of Medicine, Showa, Japan.
Int Arch Allergy Appl Immunol. 1991;96(4):322-30. doi: 10.1159/000235516.
Dialysates from Dermatophagoides farinae were partially purified. Fractionation on HPLC and anion exchange chromatography revealed that the dialysates consisted of 5 major fractions of glycoprotein whose apparent molecular sizes were 5.1, 4.1, 3.2, and less than 1.35 kD on HPLC. The apparent molecular size of two fractions was 5.3 and 2.9 kD on SDS-PAGE. They were basic glycoproteins which had a pI ranging from 7.46 to 8.71 on PAG-IEF. These fractions were allergenic in the RAST and ELISA inhibition tests but not in the skin prick test (SPT). Our results suggest that the dialysates from D. farinae have haptenic properties. The dialysates from D. farinae (low molecular weight) and its 5 fractions bound noncovalently to human serum albumin (HSA) at the free tyrosine residues of HSA. They proved to bind noncovalently to serum proteins and collagens. Once they bound to proteins, the conjugates became allergenic not only in the RAST and ELISA inhibition test but also in the SPT. Our results provide evidence that the dialysates from D. farinae have haptenic properties.
从粉尘螨中提取的透析液经过了部分纯化。通过高效液相色谱（HPLC）和阴离子交换色谱进行分级分离，结果显示透析液由5种主要的糖蛋白组分组成，在HPLC上其表观分子大小分别为5.1、4.1、3.2和小于1.35kD。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上，其中两种组分的表观分子大小为5.3和2.9kD。它们是碱性糖蛋白，在聚丙烯酰胺凝胶等电聚焦（PAG-IEF）上的等电点（pI）范围为7.46至8.71。这些组分在放射变应原吸附试验（RAST）和酶联免疫吸附测定（ELISA）抑制试验中具有变应原性，但在皮肤点刺试验（SPT）中则没有。我们的结果表明，来自粉尘螨的透析液具有半抗原特性。来自粉尘螨的透析液（低分子量）及其5种组分在人血清白蛋白（HSA）的游离酪氨酸残基处与HSA非共价结合。它们被证明能与血清蛋白和胶原蛋白非共价结合。一旦它们与蛋白质结合，结合物不仅在RAST和ELISA抑制试验中，而且在SPT中都变得具有变应原性。我们的结果提供了证据，证明来自粉尘螨的透析液具有半抗原特性。
