Dale S, Landmark E
Allergy. 1984 Nov;39(8):572-85. doi: 10.1111/j.1398-9995.1984.tb01977.x.
The allergens of a crude D. farinae extract were identified by means of crossed radioimmunoelectrophoresis (CRIE). A total of 11 allergens were identified. They were characterized as three major, three intermediate and five minor allergens, based on radiostaining in CRIE. In addition to the radiostaining due to allergens, some misleading radiostaining was observed associated with some of the other antigens. A purified commercially available preparation, Spectralgen Mite D. farinae, was compared with the crude extract by means of immunoelectrophoretic techniques and radioallergosorbent test (RAST) based techniques. The purified preparation was found to be fully representative of the allergens in the crude extract. All 11 allergens were identified in the purified preparation. The purification ratio of the allergens varied from 2 to 7, while the total specific allergenic activity was approximately 6 times higher in the purified preparation than in the crude extract, as judged from RAST-inhibition studies.
通过交叉放射免疫电泳（CRIE）对粗制粉螨提取物的变应原进行了鉴定。共鉴定出11种变应原。根据CRIE中的放射染色，它们被分为三种主要变应原、三种中等变应原和五种次要变应原。除了变应原引起的放射染色外，还观察到一些与其他一些抗原相关的误导性放射染色。通过免疫电泳技术和基于放射变应原吸附试验（RAST）的技术，将市售纯化制剂Spectralgen粉螨与粗提取物进行了比较。发现该纯化制剂完全代表了粗提取物中的变应原。在纯化制剂中鉴定出了所有11种变应原。变应原的纯化率在2至7之间，而根据RAST抑制研究判断，纯化制剂中的总特异性变应原活性比粗提取物中高约6倍。
