Wada Yasunobu, Tanaka Hideaki, Yamashita Eiki, Kubo Chikako, Ichiki-Uehara Tamaki, Nakazono-Nagaoka Eiko, Omura Toshihiro, Tsukihara Tomitake
Institute for Protein Research, Osaka University, 3-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):8-13. doi: 10.1107/S1744309107066481. Epub 2007 Dec 20.
The structure of melon necrotic spot virus (MNSV) was determined at 2.8 A resolution. Although MNSV is classified into the genus Carmovirus of the family Tombusviridae, the three-dimensional structure of MNSV showed a higher degree of similarity to tomato bushy stunt virus (TBSV), which belongs to the genus Tombusvirus, than to carnation mottle virus (CMtV), turnip crinkle virus (TCV) or cowpea mottle virus (CPMtV) from the genus Carmovirus. Thus, the classification of the family Tombusviridae at the genus level conflicts with the patterns of similarity among coat-protein structures. MNSV is one of the viruses belonging to the genera Tombusvirus or Carmovirus that are naturally transmitted in the soil by zoospores of fungal vectors. The X-ray structure of MNSV provides us with a representative structure of viruses transmitted by fungi.
甜瓜坏死斑病毒（MNSV）的结构在2.8埃分辨率下得以确定。尽管MNSV被归类于番茄病毒科中的香石竹斑驳病毒属，但MNSV的三维结构与属于番茄病毒属的番茄丛生矮缩病毒（TBSV）的相似度，高于与香石竹斑驳病毒属的香石竹斑驳病毒（CMtV）、芜菁皱缩病毒（TCV）或豇豆斑驳病毒（CPMtV）的相似度。因此，番茄病毒科在属水平上的分类与衣壳蛋白结构之间的相似模式存在冲突。MNSV是由真菌载体游动孢子在土壤中自然传播的属于番茄病毒属或香石竹斑驳病毒属的病毒之一。MNSV的X射线结构为我们提供了由真菌传播的病毒的代表性结构。
