纳升结晶实验的半自动微量接种
Semi-automated microseeding of nanolitre crystallization experiments.
作者信息
Walter Thomas S, Mancini Erika J, Kadlec Jan, Graham Stephen C, Assenberg René, Ren Jingshan, Sainsbury Sarah, Owens Raymond J, Stuart David I, Grimes Jonathan M, Harlos Karl
机构信息
Oxford Protein Production Facility and Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, England.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):14-8. doi: 10.1107/S1744309107057260. Epub 2007 Dec 20.
Abstract
A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described.
摘要
本文描述了一种用于纳升结晶实验的简单半自动微量接种程序。首先，使用聚四氟乙烯珠从微晶制备微量种子储备溶液。然后制备该微量种子储备溶液的稀释系列，并以100 nl液滴的形式分配到96孔结晶板中，便于将接种纳入高通量结晶流程。这种基本的微量接种程序已被修改，以包括添加剂筛选和交叉接种方法。文中描述了成功使用这些技术的五个实例。
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