Sukhareva N N, Uriniuk V M, Viadro M M, Navashin S M
Izv Akad Nauk SSSR Biol. 1991 Nov-Dec(6):838-56.
Novel biologically active substances were obtained from nonpathogenic for humans and free-living protozoa: Crithidia oncopelti, Trypanosoma lewisi, and Astasia longa. There were studied conditions of biosynthesis, composition and biological activity of the total lipid fraction from the protozoa species, sucrose ethers and fatty acids, the latter were isolated from A. longa--(astazilide preparation), reserve beta-1,3 glucan from A. longa (astazian preparation), and fraction of surface glycophospholipids and peptides from Crithidia oncopelti (GLP preparation). Two of three preparations studied (astazilide and GLP) are the complexes of natural substances with certain composition. Conditions of the protozoa cultivation were developed to provide standardization of the complex composition. Division of the complexes into separate components decreased or abolished the biological activity. It was established that the substances studied modify biological reactions, that is exert a systemic effect. They may be used for inhibiting the growth of experimental tumors and preventing the metastatic spreading in tumor-carrier animals. The substances obtained belong to the group of nonspecific stimulators for cellular immunity which can be used in medicine, veterinary medicine, food industry, pharmacologic industry and cosmetology.
红蝽锥虫、路氏锥虫和长尾眼虫。研究了这些原生动物物种总脂质部分、蔗糖醚和脂肪酸的生物合成条件、组成及生物活性，脂肪酸是从长尾眼虫中分离得到的（阿斯塔齐利德制剂），储备β-1,3-葡聚糖是从长尾眼虫中得到的（阿斯塔齐安制剂），表面糖磷脂和肽部分是从红蝽锥虫中得到的（GLP制剂）。所研究的三种制剂中的两种（阿斯塔齐利德和GLP）是具有特定组成的天然物质复合物。开发了原生动物培养条件以实现复合物组成的标准化。将复合物分离成单独成分会降低或消除其生物活性。已确定所研究的物质可改变生物反应，即发挥全身作用。它们可用于抑制实验性肿瘤的生长并防止肿瘤携带动物发生转移扩散。所获得的物质属于细胞免疫非特异性刺激剂类别，可用于医学、兽医学、食品工业、制药工业和美容学。
