Howell D S, Muniz O, Pita J C, Enis J E
Arthritis Rheum. 1976 May-Jun;19 Suppl 3:488-94. doi: 10.1002/1529-0131(197605/06)19:3+<488::aid-art1780190724>3.0.co;2-l.
Incubates of articular cartilage from young but not mature rabbits, as well as growth plate cartilage, elaborated PPi into basel Eagle's medium during a 4-hour period. Control rabbit synovial membrane and ear cartilage elaborated negligible amounts of PPi. The PPi was shown to be nondialyzable but could be dissociated from the alkaline phosphatase by ultracentrifugation. In 16 patients with osteoarthritis a substantial output of PPi by samples of articular cartilage from the knee was demonstrated. The present authors believe that either rapid cell division and matrix synthesis found in the base of ulcerating osteoarthritic cartilage or remodeling calcified sites comprise the origin of the PPi in such osteoarthritic cartilage. They further theorize that this PPi output accounts for a considerable fraction, if not all, of the elevated PPi levels found in synovial fluid of patients with osteoarthritis.
幼年而非成年兔的关节软骨以及生长板软骨的孵育物，在4小时内将焦磷酸（PPi）分泌到基础伊格尔培养基中。对照兔的滑膜和耳软骨分泌的PPi量可忽略不计。PPi不能通过透析去除，但可通过超速离心与碱性磷酸酶分离。在16例骨关节炎患者中，膝关节的关节软骨样本显示出大量的PPi分泌。本文作者认为，溃疡性骨关节炎软骨底部快速的细胞分裂和基质合成，或钙化部位的重塑，构成了此类骨关节炎软骨中PPi的来源。他们进一步推测，这种PPi分泌即使不能解释骨关节炎患者滑液中升高的PPi水平的全部，也占了相当大的比例。
