Boivin G, Lagier R
Virchows Arch A Pathol Anat Histopathol. 1983;400(1):13-29. doi: 10.1007/BF00627005.
A study was made by transmission electron microscopy of tissue specimens (cartilage, meniscus and synovial membrane) taken from 5 knees presenting radiological and anatomical signs of articular chondrocalcinosis and osteoarthritis. It was part of a broader study which included analysis of the same specimens by macroscopy and light microscopy as well as by X-ray diffraction of the mineral deposits. In cartilage and meniscus juxta-cellular calcium pyrophosphate dihydrate (CPPD) crystals of variable sizes were observed in the extracellular organic matrix, independent of the collagen fibrils. They occurred mainly in the superficial and middle zones but could also be seen intermingled with the apatite crystals in the cartilage calcified zone. In synovial membrane most of the CPPD crystals were extracellular but some of them could be seen in cytoplasmic phagocytic vacuoles. These observations are in agreement with those in the literature regarding the ultrastructural picture of chondrocalcinosis and support the thesis that the crystals originate in the cartilage and are phagocytized in the synovial membrane. Although the results of the present study do not provide direct evidence of a relationship between chondrocalcinosis and osteoarthritis, the data of the ultrastructural investigation appear nevertheless of great interest as a complement to the data furnished by light microscopy.
对取自5个膝关节的组织标本（软骨、半月板和滑膜）进行了透射电子显微镜研究，这些膝关节呈现出关节软骨钙质沉着症和骨关节炎的放射学及解剖学特征。这是一项更广泛研究的一部分，该研究包括通过大体检查、光学显微镜检查以及对矿物质沉积进行X射线衍射分析相同的标本。在软骨和半月板中，在细胞外有机基质中观察到大小不一的焦磷酸钙二水合物（CPPD）晶体，与胶原纤维无关。它们主要出现在表层和中层区域，但在软骨钙化区也可见与磷灰石晶体混合存在。在滑膜中，大多数CPPD晶体位于细胞外，但其中一些可见于细胞质吞噬泡中。这些观察结果与文献中关于软骨钙质沉着症超微结构图像的观察结果一致，并支持晶体起源于软骨并在滑膜中被吞噬的论点。尽管本研究结果未提供软骨钙质沉着症与骨关节炎之间关系的直接证据，但超微结构研究的数据作为光学显微镜提供的数据的补充，似乎仍然非常有趣。
