Wong H, Chang T M
Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, PQ, Canada.
Biomater Artif Cells Immobilization Biotechnol. 1991;19(4):675-86. doi: 10.3109/10731199109117846.
Standard alginate-polylysine microcapsules containing isolated rat hepatocytes were prepared. These capsules were intraperitoneally implanted into mice, and retrieved after seven days. Histological sections of the recovered microcapsules showed peritoneal lymphocyte and macrophage infiltration. Additional microscopic observations at various stages of the microencapsulation procedure, and histological observations of control non-implanted microcapsules; illustrate that encapsulated cells became embedded within the microcapsular membrane matrix. The microcapsular membrane at these sites appeared thin and often poorly formed. The cellular infiltration into the implanted microcapsules can occur through holes developed in these thin and poorly formed areas found in the microcapsular membrane. Similar observations were seen in microcapsules prepared with 20 x 10(6) and at a lower cell concentration of 10 x 10(6) suspended cells per millilitre of sodium alginate.
制备了含有分离的大鼠肝细胞的标准海藻酸盐-聚赖氨酸微胶囊。将这些微胶囊腹腔内植入小鼠体内，并在七天后取出。回收的微胶囊的组织学切片显示有腹膜淋巴细胞和巨噬细胞浸润。在微囊化过程的各个阶段进行的额外显微镜观察以及对照未植入微胶囊的组织学观察表明，被包封的细胞嵌入微囊膜基质内。这些部位的微囊膜看起来很薄，且常常形成不佳。植入微胶囊的细胞浸润可通过微囊膜中这些薄且形成不佳区域出现的孔洞发生。在每毫升海藻酸钠中悬浮细胞浓度为20×10⁶和较低的10×10⁶时制备的微胶囊中也观察到了类似现象。
