Chang S J, Lee C H, Wang Y J
Institute of Biomedical Engineering, National Yang Ming University Shih Pai, Taipei, Taiwan, ROC.
J Biomater Sci Polym Ed. 1999;10(5):531-42. doi: 10.1163/156856299x00469.
A photosensitive polymer, alpha-phenylcinnamylideneacetylated poly(L-lysine), was synthesized and characterized. This photosensitive poly(L-lysine) had 10% of its lysine residues reacted with alpha-phenylcinnamylidene acetyl group and displayed an absorption maximum at 329 nm. The photosensitive poly(L-lysine) was used for the preparation of microcapsules. The capsules formed from this photosensitive poly(L-lysine) and alginate were strengthened significantly by light irradiation. The photo cross-linked capsular membrane was permeable to proteins with mass transfer rate in the descending order: cytochrome C, myoglobin. and serum albumin. GH3 (a rat pituitary tumor cell line) cells were encapsulated and cultured with this microencapsulation system. The cells proliferated to a density of about 4 x 10(5) cells ml(-1) in the capsules after 6 days cultivation.
合成并表征了一种光敏聚合物α-苯基肉桂叉乙酰化聚（L-赖氨酸）。这种光敏聚（L-赖氨酸）有10%的赖氨酸残基与α-苯基肉桂叉乙酰基反应，在329nm处有最大吸收峰。该光敏聚（L-赖氨酸）用于制备微胶囊。由这种光敏聚（L-赖氨酸）和藻酸盐形成的胶囊经光照后显著强化。光交联的囊膜对蛋白质具有通透性，传质速率顺序为：细胞色素C、肌红蛋白、血清白蛋白。用这种微囊化系统包封并培养了GH3（大鼠垂体肿瘤细胞系）细胞。培养6天后，细胞在胶囊中增殖至密度约为4×10⁵个细胞/毫升。
