Michaels C M, Keller J C, Stanford C M
Dows Institute of Dental Research, The University of Iowa, Iowa City 52242.
J Oral Implantol. 1991;17(2):132-9.
The objective of this research was to characterize the in vitro cellular behavior of fibroblast-like cells derived from rat periodontal ligament on commercially pure titanium surfaces which were sterilized by a variety of treatments. Following standard surface preparation protocols, the Ti specimens were sterilized by either steam autoclaving, exposure to ethylene oxide gas, exposure to ultraviolet light, or plasma-cleaning in argon for either one min or five min. Fibroblast-like cells in serum-supplemented media were incubated on the various Ti specimens for up to two h. In general, the levels of cell attachment for plasma-cleaned surfaces were significantly higher than those for steam-sterilized surfaces, but were significantly lower than the attachment levels for both the ultraviolet-treated surfaces and the tissue culture plastic control. The duration of plasma cleaning itself did not have a significant effect on the percentage of cell attachment at any time period. SEM evaluations indicated that by two h, the cellular morphology was different on the variously treated specimens. These studies indicate that the method of sterilization following implant surface preparation can affect the initial in vitro biological events of cell attachment and spreading.
本研究的目的是表征源自大鼠牙周韧带的成纤维样细胞在经过多种处理灭菌的商业纯钛表面上的体外细胞行为。按照标准的表面制备方案，钛标本通过蒸汽高压灭菌、环氧乙烷气体暴露、紫外线照射或在氩气中进行1分钟或5分钟的等离子体清洁进行灭菌。将血清补充培养基中的成纤维样细胞在各种钛标本上孵育长达2小时。一般来说，等离子体清洁表面的细胞附着水平显著高于蒸汽灭菌表面，但显著低于紫外线处理表面和组织培养塑料对照的附着水平。等离子体清洁的持续时间本身在任何时间段对细胞附着百分比均无显著影响。扫描电子显微镜评估表明，到2小时时，不同处理标本上的细胞形态不同。这些研究表明，种植体表面制备后的灭菌方法会影响细胞附着和铺展的初始体外生物学事件。
