Patti L, Swinburn B, Riccardi G, Rivellese A A, Howard B V
Institute of Internal Medicine and Metabolic Diseases, 2nd Medical School, University Federico II, Naples, Italy.
Atherosclerosis. 1991 Nov;91(1-2):15-23. doi: 10.1016/0021-9150(91)90183-4.
Lipid and apoprotein composition of four very low density lipoprotein (VLDL) subfractions decreasing in Sf value were evaluated in the fasting state in 12 normolipidemic Pima Indians (6 M, 6 F, age 39 +/- 1.7 yrs) (mean +/- SEM) with non-insulin-dependent diabetes mellitus (NIDDM) in poor glycemic control (HbA1 9.8 +/- 2.9%) and in 14 normoglycemic Pima controls matched for age, BMI and lipid values. Total cholesterol (CHOL), triglyceride (TG), phospholipids (PL), total protein (TP), apo B, apo CII, apo CIII and apoE were assayed in total VLDL and in each of the four VLDL subfractions designed as A (Sf greater than 400), B (Sf 175-400), C (Sf 100-175), and D (Sf 20-100). Diabetics compared to nondiabetics had higher concentrations of all constituents of VLDL D, with the largest changes being in TG (38.0 +/- 3.8 vs 28.0 +/- 2.5 mg/dl, P less than 0.04), PL (14.0 +/- 1.3 vs 10.0 +/- 1.0 mg/dl, P less than 0.04), TP (9.8 +/- 0.8 vs 7.6 +/- 2.4 mg/dl, P less than 0.05), apo B (6.3 +/- 0.5 vs 4.7 +/- 0.4 mg/dl, P less than 0.03) and apoE (0.73 +/- 0.09 vs 0.52 +/- 0.04 mg/dl, P less than 0.04). Since no difference was found between the groups in percentage composition of lipids or apoproteins in total VLDL and in all VLDL subfractions, the data suggest that in diabetics, even when normolipidemic, there is an increase in the number rather than in the composition of the smallest VLDL subfraction (VLDL D), which are usually considered to be more atherogenic.
在空腹状态下，对12名血糖控制不佳（糖化血红蛋白A1为9.8±2.9%）的非胰岛素依赖型糖尿病（NIDDM）的正常血脂皮马印第安人（6名男性，6名女性，年龄39±1.7岁）（均值±标准误）以及14名年龄、体重指数和血脂值相匹配的血糖正常的皮马对照者，评估了四种Sf值递减的极低密度脂蛋白（VLDL）亚组分的脂质和载脂蛋白组成。对总VLDL以及设计为A（Sf大于400）、B（Sf 175 - 400）、C（Sf 100 - 175）和D（Sf 20 - 100）的四种VLDL亚组分分别测定了总胆固醇（CHOL）、甘油三酯（TG）、磷脂（PL）、总蛋白（TP）、载脂蛋白B、载脂蛋白CII、载脂蛋白CIII和载脂蛋白E。与非糖尿病患者相比，糖尿病患者的VLDL D所有成分浓度更高，变化最大的是TG（38.0±3.8对28.0±2.5mg/dl，P＜0.04）、PL（14.0±1.3对10.0±1.0mg/dl，P＜0.04）、TP（9.8±0.8对7.6±2.4mg/dl，P＜0.05）、载脂蛋白B（6.3±0.5对4.7±0.4mg/dl，P＜0.03）和载脂蛋白E（0.73±0.09对0.52±0.04mg/dl，P＜0.04）。由于在总VLDL和所有VLDL亚组分的脂质或载脂蛋白百分比组成上，两组之间未发现差异，数据表明，在糖尿病患者中，即使血脂正常，最小的VLDL亚组分（VLDL D）的数量增加而非组成增加，而VLDL D通常被认为更具致动脉粥样硬化性。
