Goromaru T, Ikejiri H, Hashimoto K, Matsuki Y
Faculty of Pharmacy and Pharmaceutical Sciences, University of Fukuyama, Japan.
Yakugaku Zasshi. 1991 Oct;111(10):612-6. doi: 10.1248/yakushi1947.111.10_612.
Quantitative analyses of iproniazid (IPN) and deuterated analogue (IPN-d6) and of isopropylhydrazine (IP-Hy) and deuterated analogue (IP-Hy-d6) after conversion to pyrazole derivatives (IDP) were carried out by gas chromatography. The complete separation of protio- from deutero-forms of IPN and IDP was achieved by using a fused-silica CBP1 capillary column (50 m). The resolution coefficients between two isotopic molecules were 1.10 for IPN and 1.62 for IDP, respectively. The present isotopic fractionation procedure was applied to the isotope dilution analyses of IPN and IP-Hy. By the measurement of the samples prepared by the addition of known amounts of IPN and IPN-d6 to the control plasma and urine of rat, a linear relationship between peak height ratio and added amount ratio was observed. The correlation coefficients obtained by regression analysis were 0.9990 for the plasma and 0.9999 for the urine, respectively. In the case of IP-Hy, a linear relationship was also observed, and the correlation coefficients were 0.9998 for the plasma and 0.9997 for the urine, respectively. The present method was compared with the gas chromatography-mass spectrometry method in urinary samples from rats treated with IPN. The results of these parallel determinations were comparable.
通过气相色谱法对异烟肼（IPN）及其氘代类似物（IPN-d6）以及异丙肼（IP-Hy）及其氘代类似物（IP-Hy-d6）转化为吡唑衍生物（IDP）后的情况进行了定量分析。使用熔融石英CBP1毛细管柱（50 m）实现了IPN和IDP的原形式与氘代形式的完全分离。两种同位素分子之间的分辨率系数，IPN为1.10，IDP为1.62。目前的同位素分馏程序应用于IPN和IP-Hy的同位素稀释分析。通过测量向大鼠对照血浆和尿液中添加已知量的IPN和IPN-d6制备的样品，观察到峰高比与添加量比之间呈线性关系。回归分析得到的血浆相关系数为0.9990，尿液相关系数为0.9999。对于IP-Hy，也观察到线性关系，血浆相关系数为0.9998，尿液相关系数为0.9997。将本方法与气相色谱-质谱法在接受IPN治疗的大鼠尿液样品中进行了比较。这些平行测定的结果具有可比性。
