Hoffman D J, Porter W R
J Chromatogr. 1983 Sep 9;276(2):301-9. doi: 10.1016/s0378-4347(00)85096-3.
Quantitation of valproic acid and a deuterated analogue in the same plasma sample by capillary gas chromatography without mass spectrometry was illustrated. Specificity was accomplished solely with a 60 m X 0.25 mm fused silica WCOT column coated with OV-351. A hexadeutero and two tetradeutero analogues of valproic acid had resolutions of at least 1.2 from valproic acid. Plasma samples were extracted with carbon tetrachloride following the addition of 2-ethylhexanoic acid as the internal standard. The method is sensitive to at least 0.5 microgram/ml and provides the capability of conducting absolute bioavailability and pulsed dosing studies with deuterated drug analogues without a mass spectrometer. The technique was applied to the analysis of plasma samples from dogs simultaneously administered valproic acid and a deuterated analogue.
阐述了在不使用质谱仪的情况下，通过毛细管气相色谱法对同一血浆样本中的丙戊酸和氘代类似物进行定量分析。特异性仅通过一根60米×0.25毫米涂覆有OV - 351的熔融石英WCOT柱来实现。丙戊酸的六氘代和两个四氘代类似物与丙戊酸的分离度至少为1.2。在加入2 - 乙基己酸作为内标后，用四氯化碳萃取血浆样本。该方法对至少0.5微克/毫升的浓度敏感，并且能够在不使用质谱仪的情况下，使用氘代药物类似物进行绝对生物利用度和脉冲给药研究。该技术应用于对同时给予丙戊酸和氘代类似物的犬类血浆样本的分析。
