Goromaru T, Maeda H
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Japan.
Biol Pharm Bull. 1994 Dec;17(12):1635-9. doi: 10.1248/bpb.17.1635.
Isotopic fractionation of isopropylantipyrine (IPA) and its deuterated analogues was examined by gas chromatography (GC) using capillary column. The separation of IPA and seven kinds of deuterated IPAs were proportional to the number of labeled deuterium atoms and inversely to the temperature of the column oven. The resolution coefficient between IPA and IPA-3-C2H3-4-(C2H3)2 (IPA-2H-7) was 1.46 at 200 degrees C for column temperature. The present isotopic fractionation procedure was applied to the isotope dilution analysis of IPA. Measurement of the sample prepared by the addition of a known amount of IPA and IPA-2H-7 to the control plasma of rabbit allowed observation of a linear relationship between peak area ratio and added amount ratio. The correlation coefficient obtained by regression analysis was 1.000. The present method was also applied to determine the plasma level of IPA in rabbit after oral administration.
采用毛细管柱气相色谱法（GC）研究了异丙安替比林（IPA）及其氘代类似物的同位素分馏。IPA与七种氘代IPA的分离与标记氘原子数成正比，与柱温成反比。在柱温200℃时，IPA与IPA-3-C2H3-4-(C2H3)2（IPA-2H-7）之间的分离系数为1.46。将目前的同位素分馏方法应用于IPA的同位素稀释分析。通过向兔对照血浆中加入已知量的IPA和IPA-2H-7制备样品，测量发现峰面积比与添加量比之间呈线性关系。回归分析得到的相关系数为1.000。该方法还应用于测定兔口服给药后血浆中IPA的水平。
