Moseley M A, Deterding L J, Tomer K B, Jorgenson J W
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Rapid Commun Mass Spectrom. 1989 Mar;3(3):87-93. doi: 10.1002/rcm.1290030310.
An on-line coaxial continuous-flow capillary-zone electrophoresis/fast-atom bombardment mass spectrometry (CZE/FAB-MS) interface is described. This interface is shown to be capable of acquiring mass spectra in an on-line fashion from low femtomole amounts of peptides while maintaining high (hundreds of thousands of plates) electrophoretic separation efficiencies. Active electrophoretic transport of the analytes directly to the FAB probe tip obviates the need for a transfer line from the end of the CZE capillary to this point, and thereby precludes the zone broadening that would otherwise occur both within such a transfer line and in the connections between the CZE column and the transfer line. The capability of acquiring an on-line tandem mass spectrometry (MS/MS) spectrum of an electrophoretically separated analyte using this interface is also demonstrated.
本文描述了一种在线同轴连续流毛细管区带电泳/快原子轰击质谱（CZE/FAB-MS）接口。该接口能够以在线方式从低飞摩尔量的肽中获取质谱，同时保持高（数十万理论塔板数）的电泳分离效率。将分析物直接主动电泳传输至FAB探针尖端，无需从CZE毛细管末端到该点的传输线，从而避免了在这样的传输线内以及CZE柱与传输线之间的连接处可能发生的区带展宽。还展示了使用该接口获取电泳分离分析物的在线串联质谱（MS/MS）谱的能力。
