Ii T, Ohashi Y, Nunomura S, Ogawa T, Nagai Y
Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Saitama.
J Biochem. 1995 Sep;118(3):526-33.
Structural characterization of mono-, di-, and trisulfated trisaccharides by fast atom bombardment (FAB) and electrospray ionization (ESI) mass spectrometry is described. Both FAB and ESI collision-induced dissociation tandem mass spectrometry (CID-MS/MS) of sulfated trisaccharides allowed identification of the sulfated sugar and provided information on the saccharide chain sequence. The ESI CID-MS/MS of multiple-charged ions provided even more structural information, and some of the useful daughter ions appeared in the higher mass region than the precursor owing to a lower charge-state. These methodologies can be applied to the structural analyses of glycoconjugates with much larger molecular masses and higher polarity, such as the poly-sulfated analogs.
本文描述了通过快原子轰击（FAB）和电喷雾电离（ESI）质谱法对单硫酸化、二硫酸化和三硫酸化三糖进行结构表征。硫酸化三糖的FAB和ESI碰撞诱导解离串联质谱（CID-MS/MS）均可鉴定硫酸化糖，并提供有关糖链序列的信息。多电荷离子的ESI CID-MS/MS提供了更多的结构信息，并且由于较低的电荷态，一些有用的子离子出现在比前体更高的质量区域。这些方法可应用于具有更大分子量和更高极性的糖缀合物的结构分析，例如多硫酸化类似物。
