Yefenof E, Klein G, Jondal M, Oldstone M B
Int J Cancer. 1976 Jun 15;17(6):693-700. doi: 10.1002/ijc.2910170602.
Receptors for the third component of complement (C3) were demonstrated on the surface of established human lymphoid cell lines by a membrane fluorescence test with FITC- or TRITC-conjugated antibodies against human C3. Two-color fluorescence staining of EBV receptors and C3 receptors showed complete overlapping of green and red fluorescence. Capping of the EBV receptor induced co-capping of the C3 receptor and vice versa. There was neither overlapping nor co-capping when EBV or C3 receptors were examined in relation to Fc receptors, surface IgM or beta2 microglobulin. The kinetic pattern of EBV receptor capping was identical with the pattern of C3 receptor capping but differed from the pattern of IgM capping. These results suggest a close association between EBV and C3 receptors on the human B-lymphocyte.
通过用异硫氰酸荧光素（FITC）或四甲基异硫氰酸罗丹明（TRITC）偶联的抗人C3抗体进行膜荧光试验，在已建立的人淋巴细胞系表面证实了补体第三成分（C3）的受体。EB病毒受体和C3受体的双色荧光染色显示绿色和红色荧光完全重叠。EB病毒受体的帽化诱导了C3受体的共帽化，反之亦然。当将EB病毒或C3受体与Fc受体、表面IgM或β2微球蛋白相关联进行检测时，既没有重叠也没有共帽化现象。EB病毒受体帽化的动力学模式与C3受体帽化的模式相同，但与IgM帽化的模式不同。这些结果表明人B淋巴细胞上的EB病毒和C3受体之间存在密切关联。
