Tedder T F, Weis J J, Clement L T, Fearon D T, Cooper M D
J Clin Immunol. 1986 Jan;6(1):65-73. doi: 10.1007/BF00915366.
A panel of monoclonal antibodies and ligands that bind to the CR1 or CR2 complement receptors of B cells has been used to investigate the role of these membrane molecules in regulating B-cell proliferation and differentiation. When CR2 was modulated from the surface of B cells by treatment with the HB-5 antibody and a secondary goat anti-mouse immunoglobulin antibody, Epstein-Barr virus-induced polyclonal B-cell proliferation and immunoglobulin production were inhibited by 83 and 90%, respectively. In contrast, modulation of other cell surface molecules, HB-2, B1, and the C3b receptor (CR1), or pretreatment of B cells with C3d,g (a CR2 ligand) or HB-5 antibody, alone minimally inhibited these responses. Neither the HB-5 antibody C3d,g, nor a monoclonal antibody (YZ-1) reactive with CR1 induced resting B cells to proliferate, nor did they alter anti-mu antibody-induced proliferation. Similarly, treatment with C3d,g or with the HB-5 or YZ-1 antibodies did not induce B cells to secrete immunoglobulin or affect pokeweed mitogen-induced plasma-cell formation. Whereas CR2 appears to be the functionally relevant receptor for Epstein-Barr virus on B cells, the effects of ligand interactions with CR1 and CR2 on normal B-cell proliferation or differentiation remain unidentified.
一组与B细胞的CR1或CR2补体受体结合的单克隆抗体和配体已被用于研究这些膜分子在调节B细胞增殖和分化中的作用。当用HB - 5抗体和二抗山羊抗小鼠免疫球蛋白抗体处理从B细胞表面调节CR2时，爱泼斯坦 - 巴尔病毒诱导的多克隆B细胞增殖和免疫球蛋白产生分别被抑制了83%和90%。相比之下，其他细胞表面分子HB - 2、B1和C3b受体（CR1）的调节，或单独用C3d,g（一种CR2配体）或HB - 5抗体对B细胞进行预处理，对这些反应的抑制作用极小。HB - 5抗体C3d,g或与CR1反应的单克隆抗体（YZ - 1）既不能诱导静息B细胞增殖，也不会改变抗μ抗体诱导的增殖。同样，用C3d,g或HB - 5或YZ - 1抗体处理也不会诱导B细胞分泌免疫球蛋白或影响商陆有丝分裂原诱导的浆细胞形成。虽然CR2似乎是B细胞上爱泼斯坦 - 巴尔病毒的功能相关受体，但配体与CR1和CR2相互作用对正常B细胞增殖或分化的影响仍不清楚。
