Einarsson K, Gustafsson J A, Ihre T, Ingelman-Sundberg M
J Clin Endocrinol Metab. 1976 Jul;43(1):56-63. doi: 10.1210/jcem-43-1-56.
The hydroxylation of steroid sulfates has been studied in liver microsomal preparations from adult humans. Ten different C18, C19, and C21 steroid sulfates and the corresponding unconjugated steroids were used as substrates. In several cases it was found that steroid sulfates were efficiently hydroxylated in a way that differed both qualitatively and quantitatively from the hydroxylation of the corresponding unconjugated substrates. Only the hydrophobic (nonsulfurylated) end of the steroid sulfate molecule was hydroxylated. The steroids sulfurylated in position 3 were generally better substrates for the liver microsomal hydroxylase system than those sulfurylated in position 17. The findings that unconjugated and sulfoconjugated steroids are metabolized along different pathways in the liver may be of general significance. Sulfoconjugation and subsequent hydroxylation may also be an important pathway in the metabolism of xenobiotics in man.
已在成年人类肝脏微粒体制剂中研究了甾体硫酸盐的羟基化作用。使用了十种不同的C18、C19和C21甾体硫酸盐以及相应的未结合甾体作为底物。在若干情况下发现，甾体硫酸盐以在定性和定量上均不同于相应未结合底物羟基化的方式被有效羟基化。仅甾体硫酸盐分子的疏水（未硫酸化）末端被羟基化。在3位硫酸化的甾体通常比在17位硫酸化的甾体是肝脏微粒体羟化酶系统更好的底物。未结合和硫酸结合甾体在肝脏中沿不同途径代谢这一发现可能具有普遍意义。硫酸结合及随后的羟基化也可能是人类异源生物代谢中的一条重要途径。
