Avichezer D, Gilboa-Garber N
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Toxicon. 1991;29(11):1305-13. doi: 10.1016/0041-0101(91)90117-a.
Examination of the in vitro effects of PA-I and PA-II lectins of Pseudomonas aeruginosa on Lewis lung carcinoma cells revealed that these lectins differ in their effects. PA-I, the galactophilic lectin, exhibited both cytotoxic and cytostatic activities on these cells (tested by [3H]thymidine incorporation and by crystal violet vital staining). The two activities were dose and time dependent and inhibitable by the addition of methyl-alpha-D-galactoside to the culture medium. PA-II, the L-fucose and D-mannose binding lectin of the same Pseudomonas strain did not exhibit such a direct toxic effect on the tumor cells but affected them in the presence of splenocytes. Its addition to the tumor cells cocultured with murine (C57B1) splenocytes led to a profound cytolysis of the tumor cells, an effect which was inhibited by L-fucose.
对铜绿假单胞菌的PA-I和PA-II凝集素对Lewis肺癌细胞的体外作用进行检测后发现，这些凝集素的作用有所不同。PA-I是嗜半乳糖凝集素，对这些细胞表现出细胞毒性和细胞生长抑制活性（通过[3H]胸腺嘧啶核苷掺入法和结晶紫活细胞染色法检测）。这两种活性均呈剂量和时间依赖性，且在培养基中添加甲基-α-D-半乳糖苷可抑制其活性。PA-II是同一铜绿假单胞菌菌株的L-岩藻糖和D-甘露糖结合凝集素，对肿瘤细胞未表现出这种直接毒性作用，但在脾细胞存在的情况下会对肿瘤细胞产生影响。将其添加到与小鼠（C57B1）脾细胞共培养的肿瘤细胞中会导致肿瘤细胞发生严重的细胞溶解，L-岩藻糖可抑制这种作用。
