Bhat M K, Prasad B N, Gowda T V
Department of Studies in Biochemistry, University of Mysore, India.
Toxicon. 1991;29(11):1345-9. doi: 10.1016/0041-0101(91)90121-7.
Snake venoms contain multimolecular forms of phospholipase A2 which are diverse with respect to their pharmacological properties. A neurotoxic PLA2 from Naja naja naja venom has been purified in two steps. (1) The whole venom was fractionated on CM-Sephadex C-25 column; 4.6% of the total PLA2 activity recovered was found in the NN-V fraction. (2) The NN-Vb-PLA2 fraction was purified to homogeneity by gel filtration of fraction NN-V on Sephadex G-50. It is a basic protein with a mol. wt between 10,500 and 11,000, and is more toxic than other basic PLA2s purified from Naja naja naja venom. The LD50 of NN-Vb-PLA2 is 0.27 mg/kg body wt. It induced neurotoxic symptoms in experimental mice and is devoid of myotoxic, anticoagulant and edema-inducing activities.
蛇毒含有多种形式的磷脂酶A2，它们在药理特性方面各不相同。已通过两步法纯化了来自眼镜蛇毒液的一种神经毒性磷脂酶A2。（1）将整个毒液在CM-葡聚糖凝胶C-25柱上进行分级分离；在NN-V级分中发现回收的总磷脂酶A2活性的4.6%。（2）通过在葡聚糖凝胶G-50上对NN-V级分进行凝胶过滤，将NN-Vb-磷脂酶A2级分纯化至同质。它是一种碱性蛋白质，分子量在10,500至11,000之间，比从眼镜蛇毒液中纯化的其他碱性磷脂酶A2毒性更大。NN-Vb-磷脂酶A2的半数致死量为0.27毫克/千克体重。它在实验小鼠中诱发神经毒性症状，且没有肌毒性、抗凝血和致水肿活性。
