印度眼镜蛇（Naja naja naja）毒液中两种有毒磷脂酶A2的比较特性研究。
Comparative characterization of two toxic phospholipases A2 from Indian cobra (Naja naja naja) venom.
作者信息
Basavarajappa B S, Gowda T V
机构信息
Department of Studies in Biochemistry, Manasagangothri, University of Mysore, India.
出版信息
Toxicon. 1992 Oct;30(10):1227-38. doi: 10.1016/0041-0101(92)90439-c.
Indian cobra venom contains many phospholipase A2 (PLA2) toxins. In the present study two toxic PLA2s have been purified from the Indian cobra (Naja naja naja) venom by column chromatography. The NN-XIa-and NN-XIb-PLA2s have mol. wts between 10,700 and 15,000. The NN-XIa-PLA2 induces myotoxic effects, oedema and neurotoxicity in mice and has an i.p. LD50 of 8.5 mg/kg body weight. The NN-XIa-PLA2 is also cytotoxic to Ehrlich ascites tumour cells. The other PLA2, NN-XIb, in contrast has an i.p. LD50 of 0.22 mg/kg body weight, and it induces acute neurotoxicity. The NN-XIb-PLA2 is devoid of the other biological activities which are exhibited by NN-XIa-PLA2.
印度眼镜蛇毒含有许多磷脂酶A2（PLA2）毒素。在本研究中，通过柱色谱法从印度眼镜蛇（Naja naja naja）毒液中纯化出两种有毒的PLA2。NN-XIa和NN-XIb-PLA2的分子量在10,700至15,000之间。NN-XIa-PLA2在小鼠中可诱导肌毒性作用、水肿和神经毒性，腹腔注射的半数致死量为8.5毫克/千克体重。NN-XIa-PLA2对艾氏腹水瘤细胞也具有细胞毒性。相比之下，另一种PLA2，即NN-XIb，腹腔注射的半数致死量为0.22毫克/千克体重，它可诱导急性神经毒性。NN-XIb-PLA2没有NN-XIa-PLA2所表现出的其他生物活性。
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