A double-label analysis demonstrating that all enkephalin-immunoreactive amacrine cells in the chicken retina express neurotensin immunoreactivity.

A previous study demonstrated less than 50% co-existence between the populations of enkephalin- and neurotensin-like immunoreactive amacrine cells in the chicken retina. The present study was undertaken with the intent of re-examining this relationship using a more sensitive double-label paradigm. An examination of retinal cryosections collected throughout the retina revealed that all labelled cells express both enkephalin and neurotensin-like immunoreactivity. Therefore, these results indicate the presence of a single population of chicken amacrine cells the members of which express both these neuropeptides.